Derivation of Breast Cancer Patient Derived Human Induced Pluripotent Stem Cell Line (YBLi006-A) with FANC-BRCA Gene Mutations: A Resource for Precision & Personalized Medicine

Swati Chitrangi; Pooja Vaity; Aishwarya Jamdar; Jaganmay Sarkar; Shweta Bhatt

doi:10.1016/j.scr.2023.103128

Derivation of Breast Cancer Patient Derived Human Induced Pluripotent Stem Cell Line (YBLi006-A) with FANC-BRCA Gene Mutations: A Resource for Precision & Personalized Medicine

Stem Cell Res. 2023 Aug:70:103128. doi: 10.1016/j.scr.2023.103128. Epub 2023 May 24.

Authors

Swati Chitrangi¹, Pooja Vaity¹, Aishwarya Jamdar¹, Jaganmay Sarkar¹, Shweta Bhatt²

Affiliations

¹ Department of Integrated Drug Discovery and Development, Yashraj Biotechnology Limited, C-232 and C-113, TTC Industrial Area, MIDC, Pawane, Navi Mumbai - 400705, India.
² Department of Integrated Drug Discovery and Development, Yashraj Biotechnology Limited, C-232 and C-113, TTC Industrial Area, MIDC, Pawane, Navi Mumbai - 400705, India; Yashraj Biotechnology GmbH, Uhlandstraße 20-25, 10623 Berlin, Germany; Yashraj Biotechnology Limited, 8, The Green STE A, Dover, Delaware State 19901, USA. Electronic address: shweta.bhatt@yashraj.com.

PMID: 37290137
DOI: 10.1016/j.scr.2023.103128

Abstract

Fanconi anemia complementation group I (FANCI) is located on the chromosome 15q26.1 locus and becomes ubiquitinated following DNA damage. 3.06% of patients with breast cancer have altered FANCI gene. We generated an iPSC line (YBLi006-A) from peripheral blood mononuclear cells (PBMCs) of a patient carrying a mutation in FANCIgene (NM_001376911.1, NM_001376910.1, NM_001113378.2; c.80G > T, c.257C > T, c.2225G > C; p.Gly27Val, p.Ala86Val, p.Cys742Ser) using non-integrating Sendai virus technology. This unique breast cancer patient-derived-iPSC line will be resourceful to analyze the entire coding sequence and splicing sites ofFANCIin high-risk familial breast cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms* / genetics
Female
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear / metabolism
Mutation / genetics
Precision Medicine