Comparative physiological and metabolomics analyses using Ag⎯NPs and HAS31 (PGPR) to alleviate Cr stress in barley (Hordeum vulgare L.)

Abstract

In the current industrial scenario, chromium (Cr) as a metal is of great importance but poses a major threat to the ecosystem because of its toxicity, but fewer studies have been conducted on its effects and alleviation strategies by using nanoparticles (NPs) and plant growth promoting rhizobacteria (PGPR). Taking into consideration the positive effects of silver⎯nanoparticles (Ag⎯NPs) and (HAS31) rhizobacteria in reducing Cr toxicity in plants, the present study was conducted. A pot experiment was conducted to determine the effects of single and/or combined application of different levels [0 (no Ag⎯NPS), 15 and 30 mM] of Ag⎯NPs and HAS31 [0 (no HAS31), 50 g and 100 g] on Cr accumulation, morpho-physiological and antioxidative defense attributes of barley (Hordeum vulgare L.) exposed to severe Cr stress [0 (without Cr stress), 50 and 100 μM)]. Results from the present study showed that the increasing levels of Cr in the soil significantly (P < 0.05) decreased plant growth and biomass, photosynthetic pigments, gas exchange attributes, sugars, and nutritional contents from the roots and shoots of the plants. In contrast, increasing levels of Cr in the soil significantly (P < 0.05) increased oxidative stress indicators in term of malondialdehyde, hydrogen peroxide, and electrolyte leakage, and also increased organic acid exudation patter in the roots of H. vulgare. Although, the activities of enzymatic antioxidants and the response of their gene expressions in the roots and shoots of the plants and non-enzymatic such as phenolic, flavonoid, ascorbic acid, and anthocyanin contents were increased by increasing the Cr concentration in the soil. The negative impacts of Cr injury were reduced by the application of PGPR (HAS31) and Ag⎯NPs, which increased plant growth and biomass, improved photosynthetic apparatus, antioxidant enzymes, and mineral uptake, as well as diminished the exudation of organic acids and oxidative stress indicators in roots of H. vulgare by decreasing Cr toxicity. Research findings, therefore, suggest that the application of PGPR (HAS31) and Ag⎯NPs can ameliorate Cr toxicity in H. vulgare, resulting in improved plant growth and composition under metal stress, as depicted by balanced exudation of organic acids.

Keywords: Cereal grain; Gene expression; Metal toxicity; Nutritional efflux; Oxidative stress.