Development of a Streptococcus pluranimalium-specific PCR assay

Yuko Kitamura; Yusuke Kusajima; Mariko Okamoto; Yuichi Ueno; Takashi Mada; Masatoshi Okura; Daisuke Takamatsu

doi:10.1016/j.mimet.2023.106766

Development of a Streptococcus pluranimalium-specific PCR assay

J Microbiol Methods. 2023 Aug:211:106766. doi: 10.1016/j.mimet.2023.106766. Epub 2023 Jun 12.

Authors

Yuko Kitamura¹, Yusuke Kusajima², Mariko Okamoto³, Yuichi Ueno³, Takashi Mada³, Masatoshi Okura⁴, Daisuke Takamatsu⁵

Affiliations

¹ Gifu Prefectural Chuo Livestock Hygiene Service Center, Gifu, Gifu 501-1112, Japan.
² Tochigi Prefectural Central Livestock Hygiene Service Center, Utsunomiya, Tochigi 321-0905, Japan.
³ Division of Infectious Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
⁴ Division of Transboundary Animal Disease Research, Kagoshima Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kagoshima, Kagoshima 891-0105, Japan.
⁵ Division of Infectious Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan; The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501-1193, Japan; Joint Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501-1193, Japan. Electronic address: p1013dt@affrc.go.jp.

PMID: 37315770
DOI: 10.1016/j.mimet.2023.106766

Abstract

Streptococcus pluranimalium, an emerging zoonotic pathogen associated with infections in various animal species and humans, cannot be reliably identified by phenotypic characterization using the commercial kits routinely used in laboratories. We herein developed the first S. pluranimalium-specific PCR assay useful for the easy and reliable identification of this species.

Keywords: PCR; Species identification; Streptococcus pluranimalium; recN.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Nucleic Acid Amplification Techniques
Polymerase Chain Reaction
Streptococcal Infections* / diagnosis
Streptococcal Infections* / veterinary
Streptococcus / genetics

Supplementary concepts

Streptococcus pluranimalium