Faraday cage-type ECL biosensor for the detection of circulating tumor cell MCF-7

Jie Zhang; Huiqian Zhou; Tingting Hao; Yiyao Yang; Qingqing Zhang; Jinyun Li; Meng Ye; Yangbo Wu; Wanlei Gao; Zhiyong Guo

doi:10.1016/j.aca.2023.341465

Faraday cage-type ECL biosensor for the detection of circulating tumor cell MCF-7

Anal Chim Acta. 2023 Aug 29:1271:341465. doi: 10.1016/j.aca.2023.341465. Epub 2023 May 31.

Authors

Jie Zhang¹, Huiqian Zhou¹, Tingting Hao¹, Yiyao Yang¹, Qingqing Zhang², Jinyun Li³, Meng Ye³, Yangbo Wu⁴, Wanlei Gao⁴, Zhiyong Guo⁵

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China. Electronic address: m15757825634@163.com.
³ The Affiliated Hospital of Medical School, Ningbo University, Ningbo, 315020, PR China.
⁴ Faculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, 315211, PR China.
⁵ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China. Electronic address: guozhiyong@nbu.edu.cn.

PMID: 37328246
DOI: 10.1016/j.aca.2023.341465

Abstract

Herein, a Faraday cage-type electrochemiluminescence biosensor was designed for the detection of human breast cancer cell MCF-7. Two kinds of nanomaterials, Fe₃O₄-APTs and GO@PTCA-APTs, were synthesized as capture unit and signal unit, respectively. In presence of the target MCF-7, the Faraday cage-type electrochemiluminescence biosensor was constructed by forming a complex "capture unit-MCF-7-signal unit". In this case, lots of electrochemiluminescence signal probes were assembled and could participate in the electrode reaction, achieving a significant increase in sensitivity. In addition, the double aptamer recognition strategy was adopted to improve the capture, enrichment efficiency and detection reliability. Under optimal experimental conditions, the limit of detection was 3 cells/mL. And, the sensor could afford the detection of actual human blood samples, which is the first report on the detection of intact circulating tumor cells by the Faraday cage-type electrochemiluminescence biosensor.

Keywords: Aptamer; Breast cancer cell MCF-7; Circulating tumor cells; Electrochemiluminescence; Faraday cage-type sensor; Signal on.

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Humans
Limit of Detection
Luminescent Measurements
MCF-7 Cells
Neoplastic Cells, Circulating*
Reproducibility of Results