Application of the wholebrain calculation interactive framework to map whole-brain neural connectivity networks

J Chem Neuroanat. 2023 Oct:132:102304. doi: 10.1016/j.jchemneu.2023.102304. Epub 2023 Jun 17.

Abstract

The aim of this work was to develop a simple and feasible method of mapping the neural network topology of the mouse brain. Wild-type C57BL/6 J mice (n = 10) aged 8-10 weeks were injected with the cholera toxin subunit B (CTB) tracer in the anterior (NAcCA) and posterior (NAcCP) parts of the nucleus accumbens (NAc) core and in the medial (NAcSM) and lateral (NAcSL) parts of the NAc shell. The labeled neurons were reconstructed using the WholeBrain Calculation Interactive Framework. The NAcCA receives neuronal projections from the olfactory areas (OLF) and isocortex; the thalamus and isocortex project more fibers to the NAcSL, and the hypothalamus send more fiber projections to the NAcSM. Cell resolution can be automatically annotated, analyzed, and visualized using the WholeBrain Calculation Interactive Framework, making large-scale mapping of mouse brains at cellular and subcellular resolutions easier and more accurate.

Keywords: Neural circuit; Retrograde tracing; WholeBrain calculation interactive framework; Wholebrain map.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Mapping
  • Brain*
  • Cholera Toxin
  • Hypothalamus*
  • Mice
  • Mice, Inbred C57BL
  • Neural Pathways / physiology
  • Nucleus Accumbens
  • Thalamus / physiology

Substances

  • Cholera Toxin