Epidemiology and zoonotic transmission of mcr-positive and carbapenemase-producing Enterobacterales on German turkey farms

Katja Nordhoff; Martina Scharlach; Natalie Effelsberg; Carolin Knorr; Dagmar Rocker; Katja Claussen; Richard Egelkamp; Alexander C Mellmann; Andreas Moss; Ilona Müller; Sarah Andrea Roth; Christiane Werckenthin; Anne Wöhlke; Joachim Ehlers; Robin Köck

doi:10.3389/fmicb.2023.1183984

Epidemiology and zoonotic transmission of mcr-positive and carbapenemase-producing Enterobacterales on German turkey farms

Front Microbiol. 2023 Jun 2:14:1183984. doi: 10.3389/fmicb.2023.1183984. eCollection 2023.

Authors

Katja Nordhoff^{1

2}, Martina Scharlach³, Natalie Effelsberg⁴, Carolin Knorr¹, Dagmar Rocker³, Katja Claussen³, Richard Egelkamp³, Alexander C Mellmann⁴, Andreas Moss¹, Ilona Müller³, Sarah Andrea Roth³, Christiane Werckenthin¹, Anne Wöhlke¹, Joachim Ehlers¹, Robin Köck^{4

5}

Affiliations

¹ Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Oldenburg, Germany.
² Perioperative Inflammation and Infection, Department of Human Medicine, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany.
³ Public Health Agency of Lower Saxony (NLGA), Hanover, Germany.
⁴ Institute of Hygiene, University Hospital Münster, Münster, Germany.
⁵ Hygiene and Environmental Medicine, University Medicine Essen, Essen, Germany.

Abstract

Introduction: The emergence of carbapenem-resistant bacteria causing serious infections may lead to more frequent use of previously abandoned antibiotics like colistin. However, mobile colistin resistance genes (mcr) can jeopardise its effectiveness in both human and veterinary medicine. In Germany, turkeys have been identified as the food-producing animal most likely to harbour mcr-positive colistin-resistant Enterobacterales (mcr-Col-E). Therefore, the aim of the present study was to assess the prevalence of both mcr-Col-E and carbapenemase-producing Enterobacterales (CPE) in German turkey herds and humans in contact with these herds.

Methods: In 2018 and 2019, 175 environmental (boot swabs of turkey faeces) and 46 human stool samples were analysed using a combination of enrichment-based culture, PCR, core genome multilocus sequence typing (cgMLST) and plasmid typing.

Results: mcr-Col-E were detected in 123 of the 175 turkey farms in this study (70.3%). mcr-Col-E isolates were Escherichia coli (98.4%) and Klebsiella spp. (1.6%). Herds that had been treated with colistin were more likely to harbour mcr-Col-E, with 82.2% compared to 66.2% in untreated herds (p = 0.0298). Prevalence also depended on husbandry, with 7.1% mcr-Col-E in organic farms compared to 74.5% in conventional ones (p < 0.001). In addition, four of the 46 (8.7%) human participants were colonised with mcr-Col-E. mcr-Col-E isolates from stables had minimum inhibitory concentrations (MICs) from 4 to ≥ 32 mg/l, human isolates ranged from 4 to 8 mg/l. cgMLST showed no clonal transmission of isolates. For one farm, plasmid typing revealed great similarities between plasmids from an environmental and a human sample. No CPE were found in turkey herds or humans.

Discussion: These findings confirm that mcr-Col-E-prevalence is high in turkey farms, but no evidence of direct zoonotic transmission of clonal mcr-Col-E strains was found. However, the results indicate that plasmids may be transmitted between E. coli isolates from animals and humans.

Keywords: colistin resistance (mcr); farmers; one health; personnel; poultry; staff; zoonosis.