Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA

Alexandria B Boehm; Marlene K Wolfe; Krista R Wigginton; Amanda Bidwell; Bradley J White; Bridgette Hughes; Dorothea Duong; Vikram Chan-Herur; Heather N Bischel; Colleen C Naughton

doi:10.1038/s41597-023-02297-7

Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA

Sci Data. 2023 Jun 22;10(1):396. doi: 10.1038/s41597-023-02297-7.

Authors

Affiliations

¹ Department of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford University, Stanford, CA, USA. aboehm@stanford.edu.
² Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA.
³ Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, 48109, Michigan, USA.
⁴ Department of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford University, Stanford, CA, USA.
⁵ Verily Life Sciences, South San Francisco, CA, USA.
⁶ Department of Civil and Environmental Engineering, University of California Davis, Davis, CA, 95616, USA.
⁷ Department of Civil and Environmental Engineering, University of California Merced, Merced, CA, 95343, USA.

Abstract

We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox virus, human metapneumovirus, norovirus GII, and pepper mild mottle virus nucleic acids in wastewater solids at twelve wastewater treatment plants in Central California, USA. Measurements were made daily for up to two years, depending on the wastewater treatment plant. Measurements were made using digital droplet (reverse-transcription-) polymerase chain reaction (RT-PCR) following best practices for making environmental molecular biology measurements. These data can be used to better understand disease occurrence in communities contributing to the wastewater.

Publication types

Dataset

MeSH terms

COVID-19
Humans
Metapneumovirus*
RNA, Viral*
Respiratory Syncytial Virus, Human*
SARS-CoV-2*
Wastewater

Substances

Wastewater
RNA, Viral