Mapping of Notch signaling in the developing organ of Corti in common marmosets

Front Neuroanat. 2023 Jun 7:17:1188886. doi: 10.3389/fnana.2023.1188886. eCollection 2023.

Abstract

Introduction: The well-regulated development of the sensory epithelium is essential for hearing. This process involves the specification of a pro-sensory epithelium containing common progenitors that differentiate into hair and supporting cells. Notch signaling is one of the most critical pathways during these processes, and its modification is thought to be a feasible approach for treating hearing loss. Despite interspecies differences between rodents and primates or humans, most of our current knowledge regarding cochlear development has been obtained from rodent models.

Methods: We therefore examined and mapped the expression patterns of Notch signal components in the developing cochlea of the common marmoset (Callithrix jacchus), a small monkey species native to the New World, a primate model animal.

Results: In contrast to the preserved expression patterns of the Notch signaling components in the hair cell differentiation between primates and rodents, we unveiled relatively large interspecies differences during the maturation of supporting cells.

Discussion: This improved knowledge of Notch signaling during primate cochlear development will facilitate the development of future regenerative therapies.

Keywords: Notch; cochlea; cochlear development; inner ear; marmoset.

Grants and funding

MH was supported by a grant from the Japanese government, MEXT KAKENHI [Grant-in-Aid for Scientific Research (B) 20H03836, Grant-in-Aid for Challenging Research (Exploratory) 21K19581], the Keio Medical Association, Keio University Medical Science Fund, the Society for Promotion of International Oto-Rhino-Laryngology (SPIO), the Kanae Foundation for the Promotion of Medical Science, the Takeda Science Foundation, and Keio Gijuku Academic Development Funds. MF was supported by a grant from the Japanese government, MEXT KAKENHI [Grant-in-Aid for Scientific Research (A) 21H04839].