Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.
Keywords: Human blastoids; Human extraembryonic cell types; Human implantation model; Hypoblast cells; Integrated models of human embryos; Naive human pluripotent stem cells; The generation of blastocyst-like structures; Trophoblast cells.
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