Interferon-induced transmembrane protein 3 in hepatocellular carcinoma patients

Rania M Bondok; Lamiaa A Barakat; Alyaa R Elsergany; Nancy Mahsoub; Maivel H Ghattas

doi:10.1186/s12885-023-11071-2

Interferon-induced transmembrane protein 3 in hepatocellular carcinoma patients

BMC Cancer. 2023 Jun 23;23(1):584. doi: 10.1186/s12885-023-11071-2.

Authors

Rania M Bondok¹, Lamiaa A Barakat², Alyaa R Elsergany³, Nancy Mahsoub⁴, Maivel H Ghattas⁵

Affiliations

¹ Department of Biochemistry, Faculty of Science, Port-Said University, Port Said, Egypt. r.bondok@sci.psu.edu.eg.
² Department of Biochemistry, Faculty of Science, Port-Said University, Port Said, Egypt.
³ Internal Medicine Department, Oncology Center, Faculty of Medicine, Mansoura University, El Mansoura, Egypt.
⁴ Department of Clinical Pathology, Faculty of Medicine, Mansoura University, El Mansoura, Egypt.
⁵ Department of Medical Biochemistry, Faculty of Medicine, Port-Said University, Port Said, Egypt.

Abstract

Objective: The study aimed to investigate the over expression of IFITM3 in hepatocellular carcinoma Egyptian patients.

Background: Hepatocellular carcinoma (HCC) continues to be a serious disease burden. Interferon Induced Transmembrane protein 3 (IFITM3) is a protein that encoded in humans by the IFITM3 gene. It plays a critical role in the immune system's defense, responsible for a large portion of the antiviral activity. In this study, we showed that IFITM3 rs 12252-CC was over expressed in HCC patients compared to control group with HCV infection.

Method: DNA sequencing was applied for detection of IFITM3 rs 12252-CC and IFITM3 protein level was measured by ELISA to 50 patients with HCC with cirrhosis and 50 with Hepatitis C virus infection.

Results: The obtained results of this study indicated that IFITM3 rs 12252-CC was significantly elevated in HCC group, the codominant model of CC genotype of IFITM3 gene had high association with risk of hepatocellular carcinoma with odd ratio (OR) = 2.70, p = 0.041.

Conclusion: IFITM3 play an important role in progression of hepatocellular carcinoma. Results revealed that IFITM3 rs 12252-CC among Hepatocellular carcinoma patients would allow diagnosis and starting intervention.

Keywords: Gene polymorphism; Hepatitis C virus; Hepatocellular carcinoma; Interferon-Induced transmembrane protein 3.

MeSH terms

Carcinoma, Hepatocellular* / pathology
Hepacivirus
Hepatitis C* / complications
Hepatitis C* / genetics
Humans
Interferons / metabolism
Liver Neoplasms* / pathology
Membrane Proteins / genetics
Membrane Proteins / metabolism
Polymorphism, Single Nucleotide
RNA-Binding Proteins / genetics

Substances

Interferons
IFITM3 protein, human
Membrane Proteins
RNA-Binding Proteins