Periosteum-derived skeletal stem cells encapsulated in platelet-rich plasma enhance the repair of bone defect

Tissue Cell. 2023 Aug;83:102144. doi: 10.1016/j.tice.2023.102144. Epub 2023 Jun 20.


Background: Spontaneous restoration of large bone defects remains a challenge under infections, tumors, and crushing conditions. Current stem cell-based therapies for treating bone defects need improvement, because the used stem cells are isolated by a traditional protocol, which is based on their properties of in-vitro plastic adherence and fibroblastic colony formation. The stem cells isolated by the traditional protocol belong to a multicellular type mixture, individual cells vary in proliferative and osteogenic potential. Thus, developing a protocol capable of isolating stem cell subset with higher purity is required and urgent.

Aim: This study aimed to sort a subpopulation of stem cells from periosteum using flow cytometry (named as FC-PSCs), and evaluate the proliferative and osteogenic capacity of FC-PSCs in-vitro, and then establish a new stem cell-based therapies for treating bone defects by delivering the FC-PSCs within platelet-rich plasma (PRP).

Methods: Mouse periosteum was used to sort FC-PSCs using flow cytometry with CD45-TER119-TIE2-ITGAV+CD90 + 6C3-CD105- markers, or isolate periosteum-derived stem cells with the traditional protocol (TP-PSCs) as control. After evaluating the FC-PSCs proliferation and osteogenic differentiation in-vitro as well as the promotive efficacy of platelet-rich plasma (PRP) on FC-PSCs proliferation and osteogenic differentiation, the FC-PSCs were delivered into the femoral epiphysis bone defect site of a mouse model by platelet-rich plasma (PRP). At postoperative 14 or 28 days, these mice were euthanized for harvest the femur specimens for micro-CT, histological evaluation.

Results: In-vitro results determined that the FC-PSCs showed more capacity for proliferation and osteogenic differentiation compared with the TP-PSCs. In addition, in-vitro results showed the promotive efficacy of PRP on FC-PSCs proliferation and osteogenic differentiation. In-vivo results showed that the FC-PSCs delivered by PRP was able to facilitate the repair of bone defects by stimulating new bone formation and remodeling.

Conclusion: FC-PSCs delivered by PRP enhance the repair of bone defects by stimulating new bone formation and remodeling.

Keywords: Bone defect; Periosteum; Platelet-rich plasma; Skeletal stem cells.

MeSH terms

  • Animals
  • Bone Regeneration
  • Cell Differentiation
  • Cell Proliferation
  • Mesenchymal Stem Cells*
  • Mice
  • Osteogenesis
  • Periosteum
  • Platelet-Rich Plasma*
  • Stem Cells