The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence

F1000Res. 2023 Jun 20:12:277. doi: 10.12688/f1000research.131852.2. eCollection 2023.

Abstract

TAR DNA-binding protein 43 (TDP-43) is a DNA/RNA binding protein playing a critical role in the regulation of transcription, splicing and RNA stability. Mutations in TARDBP leading to aggregation, are suspected to be a characteristic feature of various neurogenerative diseases. The lack of well-characterized anti- TDP-43 antibodies acts as a barrier to establish reproducible TDP-43 research. In this study, we characterized eighteen TDP-43 commercial antibodies for Western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many well-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

Keywords: TARDBP; TDP-43; Uniprot ID Q13148; Western Blot; antibody characterization; antibody validation; immunofluorescence; immunoprecipitation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Line
  • DNA-Binding Proteins* / genetics
  • Fluorescent Antibody Technique
  • Immunoprecipitation

Substances

  • DNA-Binding Proteins

Grants and funding