Structural analysis and attenuates hyperuricemic nephropathy of dextran from the Imperata cylindrica Beauv. var. major (Nees) C. E. Hubb

Wenchen Yu; Yi Xiong; Mengnan Liu; Deyong Zeng; Haitian Zhao; Jiaren Liu; Weihong Lu

doi:10.1016/j.carbpol.2023.121064

Structural analysis and attenuates hyperuricemic nephropathy of dextran from the Imperata cylindrica Beauv. var. major (Nees) C. E. Hubb

Carbohydr Polym. 2023 Oct 1:317:121064. doi: 10.1016/j.carbpol.2023.121064. Epub 2023 May 29.

Authors

Wenchen Yu¹, Yi Xiong¹, Mengnan Liu¹, Deyong Zeng¹, Haitian Zhao², Jiaren Liu³, Weihong Lu⁴

Affiliations

¹ School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; The Intelligent Equipment Research Center for the Exploitation of Characteristic Food & Medicine Resources, Chongqing Research Institute， Harbin Institute of Technology, Chongqing 401135, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
² School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; The Intelligent Equipment Research Center for the Exploitation of Characteristic Food & Medicine Resources, Chongqing Research Institute， Harbin Institute of Technology, Chongqing 401135, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China.
³ School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China.
⁴ The Intelligent Equipment Research Center for the Exploitation of Characteristic Food & Medicine Resources, Chongqing Research Institute， Harbin Institute of Technology, Chongqing 401135, China; National and Local Joint Engineering Laboratory for Synthesis, Transformation and Separation of Extreme Environmental Nutrients, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China. Electronic address: lwh@hit.edu.cn.

PMID: 37364951
DOI: 10.1016/j.carbpol.2023.121064

Abstract

ICPC-a was from the Imperata cylindrica with a molecular weight of 45 kDa, which was composed of α-D-1,3-Glcp and α-D-1,6-Glcp. The ICPC-a showed thermal stability, maintaining its structural integrity up to 220°C. X-ray diffraction analysis confirmed its amorphous nature, while scanning electron microscopy revealed a layered morphology. ICPC-a significantly ameliorated uric acid stimulation-induced HK-2 cell injury and apoptosis and reduced uric acid levels in mice with hyperuricemic nephropathy. ICPC-a protected against renal injury by inhibiting lipid peroxidation levels, increasing antioxidant damage and defense levels, inhibiting secretion of pro-inflammatory factors, regulating purine metabolism, PI3K-Akt signaling pathway, NF-κB signaling pathway, inflammatory bowel disease, mTOR signaling pathway, and MAPK signaling pathway. These findings indicate that ICPC-a is a promising natural substance with multiple targets, multiple pathways of action, and without toxicity, making it a valuable subject for further research and development.

Keywords: Hyperuricemic nephropathy; Imperata cylindrica; NMR spectrometry.

MeSH terms

Animals
Dextrans* / metabolism
Kidney / metabolism
Mice
NF-kappa B / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Poaceae / metabolism
Uric Acid*

Substances

Uric Acid
Dextrans
Phosphatidylinositol 3-Kinases
NF-kappa B