In vitro ADP-ribosylation of chromosomal proteins of the brain of developing rats

Mol Biol Rep. 1986;11(2):63-8. doi: 10.1007/BF00364815.

Abstract

In vitro ADP-ribosylation of chromosomal proteins and its modulation by spermine, 3-aminobenzamide (3-AB) and benzamide were studied by incubating the nuclei of cerebral hemisphere of 3-, 14- and 30-day old rats with 32P-NAD+. Histones get ADP-ribosylated more than the non-histone chromosomal (NHC) proteins. H1 is the major target for ADP-ribosylation. Among the nucleosomal histones, H2B is ADP-ribosylated most. The other core histones also get ADP-ribosylated to a lesser extent. ADP-ribosylation of both histones and NHC proteins decreases during development. Spermine stimulates, whereas 3-AB and benzamide inhibit, 32P-ADP-ribose incorporation into histones and NHC proteins. These effects decrease with development. Mild digestion of chromatin by micrococcal nuclease (MNase), EcoRI and AluI prior to ADP-ribosylation stimulates incorporation of 32P-ADP-ribose. The degree of stimulation decreases as development proceeds. Such alterations indicate progressive condensation of chromatin with development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate Ribose / metabolism*
  • Aging
  • Animals
  • Benzamides / pharmacology
  • Brain / growth & development
  • Brain / metabolism*
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Histones / metabolism*
  • Male
  • Micrococcal Nuclease / pharmacology
  • Nucleoside Diphosphate Sugars / metabolism*
  • Protein Processing, Post-Translational / drug effects
  • Rats
  • Rats, Inbred Strains
  • Spermine / pharmacology

Substances

  • Benzamides
  • Chromosomal Proteins, Non-Histone
  • Histones
  • Nucleoside Diphosphate Sugars
  • Adenosine Diphosphate Ribose
  • Spermine
  • benzamide
  • 3-aminobenzamide
  • Micrococcal Nuclease