Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis

Clin Epigenetics. 2023 Jun 27;15(1):105. doi: 10.1186/s13148-023-01521-w.

Abstract

Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in the genes MYO1D, STK17A, and SP140 correlated with CD34+ cell numbers in mobilized peripheral blood and with blast counts in leukemia. In the future, such epigenetic biomarkers can support the evaluation of stem cell mobilization, HSPC harvesting, or blast count in leukemia.

Keywords: Biomarker; Blast; CD34; DNA methylation; Epigenetic; HSC; Hematopoietic stem cells; Leukemia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / metabolism
  • Apoptosis Regulatory Proteins
  • DNA Methylation*
  • Granulocyte Colony-Stimulating Factor
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Leukemia* / metabolism
  • Protein Serine-Threonine Kinases

Substances

  • Granulocyte Colony-Stimulating Factor
  • Antigens, CD34
  • STK17A protein, human
  • Protein Serine-Threonine Kinases
  • Apoptosis Regulatory Proteins