Investigation of the Antifungal and Anticancer Effects of the Novel Synthesized Thiazolidinedione by Ion-Conductance Microscopy

Cells. 2023 Jun 19;12(12):1666. doi: 10.3390/cells12121666.


In connection with the emergence of new pathogenic strains of Candida, the search for more effective antifungal drugs becomes a challenge. Part of the preclinical trials of such drugs can be carried out using the innovative ion-conductance microscopy (ICM) method, whose unique characteristics make it possible to study the biophysical characteristics of biological objects with high accuracy and low invasiveness. We conducted a study of a novel synthesized thiazolidinedione's antimicrobial (for Candida spp.) and anticancer properties (on samples of the human prostate cell line PC3), and its drug toxicity (on a sample of the human kidney cell line HEK293). We used a scanning ion-conductance microscope (SICM) to obtain the topography and mechanical properties of cells and an amperometric method using Pt-nanoelectrodes to register reactive oxygen species (ROS) expression. All data and results are obtained and presented for the first time.

Keywords: Candida; HEK239; PC3; ROS level; SICM; anticancer; antifungal; cell stiffness; thiazolidinedione.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents
  • HEK293 Cells
  • Humans
  • Kidney
  • Microscopy* / methods
  • Thiazolidinediones* / pharmacology


  • Antifungal Agents
  • Thiazolidinediones

Grants and funding

The ROS electrochemical measurements were carried out within the framework of the Implementation Program Priority 2030 (NUST MISIS). The SICM measurements were supported by the Russian Science Foundation (Grant No. 221900824).