The capture, transport, and maintenance of live adult Atlantic coast squid (Loligo pealei) are described. The objective was to obtain healthy live squid from a coastal region and to maintain them at an inland research facility long enough to provide at least 4 days of electrophysiological research. An inexpensive closed aquarium system is described, which utilizes an ion-exchange resin in the filter, that allows a typical survival time of at least 4.5 days. Similar closed aquarium systems may be of interest to other biophysicists who wish to maintain live squid away from coastal research facilities.