This study investigates the development of dual-substrate mixotrophy strategy to cultivate the microalga Haematococcus lacustris for astaxanthin production. The influence of different concentrations of acetate and pyruvate on biomass productivity was first assessed individually, and then both substrates were used together to improve biomass growth in the green phase and astaxanthin accumulation in red the phase. The results showed that dual-substrates mixotrophy significantly increased the biomass productivity during green growth phase up to 2-fold compared to phototrophic controls. Furthermore, supplementation of dual-substrate to the red phase increased astaxanthin accumulation by 10% in the dual-substrate group compared to single-substrate acetate and no substrate. This dual-substrate mixotrophy approach shows promise for cultivating Haematococcus for commercial production of biological astaxanthin in indoor closed systems.
Keywords: acetate; astaxanthin; dual-substrate; mixotrophic cultivation; pyruvate.
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