γδ-T cell with high toxic potential was associated with recurrent miscarriage

Abstract

Problem: RM is a common clinical disease in reproduction, affecting approximately 1%-3% of women worldwide. Previous studies have shown the role of peripheral blood γδ-T cells during physiological pregnancy. However, the relationship between the immune status of peripheral blood γδ-T cells and RM is still not well defined.

Method of study: In this study, mid-luteal peripheral blood from 51 RM patients and 40 healthy women was collected to determine the immune status of γδ-T cells. The percentage of peripheral blood γδ-T cells, and the molecules mediating their toxic potential, including cytotoxic granules (perforin, granzyme B, and granulysin) and receptors (NKG2D, CD158a, and CD158b), were detected by flow cytometry.

Results: Compared to healthy control, an increase in the proportion of total CD3⁺ T cells in lymphocytes and a decrease in the ratio of γδ-T cells to CD3⁺ T cells were observed in patients with RM. The percentages of granzyme B⁺ γδ-T cells and CD158a⁺ γδ-T cells in total γδ-T cells or lymphocytes were significantly increased in patients with RM, compared with healthy control. Conversely, CD158b⁺ γδ-T cells in total γδ-T cells or lymphocytes were significantly decreased in the RM group.

Conclusion: Increased peripheral blood γδ-T cell with high toxic potential was associated with RM.

Keywords: CD158a receptor; CD158b receptor; recurrent miscarriage; toxic granules; γδ-T cell.