The construction of Fe-porphyrin nanozymes with peroxidase-like activity for colorimetric detection of glucose

Xiaochun Hu; Guanghua Wang; Kang Fang; Ruihao Li; Chunyan Dong; Shuo Shi; Hui Li

doi:10.1016/j.ab.2023.115224

The construction of Fe-porphyrin nanozymes with peroxidase-like activity for colorimetric detection of glucose

Anal Biochem. 2023 Aug 15:675:115224. doi: 10.1016/j.ab.2023.115224. Epub 2023 Jun 30.

Authors

Xiaochun Hu¹, Guanghua Wang², Kang Fang³, Ruihao Li³, Chunyan Dong³, Shuo Shi⁴, Hui Li⁵

Affiliations

¹ Department of Obstetrics and Gynecology, East Hospital Affiliated to Tongji University, Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China; School of Medicine, Shanghai University, Shanghai, 200444, China.
² Department of Obstetrics and Gynecology, Tongren Hospital, Shanghai Jiaotong University, School of Medicine, China. Electronic address: ghwang@tongji.edu.cn.
³ Department of Obstetrics and Gynecology, East Hospital Affiliated to Tongji University, Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China.
⁴ Department of Obstetrics and Gynecology, East Hospital Affiliated to Tongji University, Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China. Electronic address: shishuo@tongji.edu.cn.
⁵ Department of Obstetrics and Gynecology, East Hospital Affiliated to Tongji University, Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China. Electronic address: newoneday@126.com.

PMID: 37393976
DOI: 10.1016/j.ab.2023.115224

Abstract

As a type of nanomaterials with enzyme-mimetic catalytic properties, nanozymes have attracted wide concern in biological detection. H₂O₂ was the characteristic product of diverse biological reactions, and the quantitative analysis for H₂O₂ was an important way to detect disease biomarkers, such as acetylcholine, cholesterol, uric acid and glucose. Therefore, there is of great significance for developing a simple and sensitive nanozyme to detect H₂O₂ and disease biomarkers by combining with corresponding enzyme. In this work, Fe-TCPP MOFs were successfully prepared by the coordination between iron ions and porphyrin ligands (TCPP). In addition, the peroxidase (POD) activity of Fe-TCPP was proved, in detail, Fe-TCPP could catalyze H₂O₂ to produce ·OH. Herein, glucose oxidase (GOx) was chosen as the model to build cascade reaction by combining Fe-TCPP to detect glucose. The results indicated glucose could be detected by this cascade system selectively and sensitively, and the limit of detection of glucose was achieved to 0.12 μM. Furthermore, a portable hydrogel (Fe-TCPP@GEL) was further established, which encapsulated Fe-TCPP MOFs, GOx and TMB in one system. This functional hydrogel could be applied for colorimetric detection of glucose by coupling with a smartphone easily.

Keywords: Glucose; Glucose oxidase; Hydrogen peroxide; Metal organic frameworks; Nanozymes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers
Colorimetry / methods
Glucose / analysis
Glucose Oxidase
Hydrogen Peroxide
Metal-Organic Frameworks*
Metalloporphyrins
Peroxidases
Porphyrins*

Substances

Glucose
Hydrogen Peroxide
Porphyrins
Metal-Organic Frameworks
Peroxidases
Biomarkers
Glucose Oxidase
iron 5,10,15,20-tetrakis-4-carboxyphenylporphyrin
Metalloporphyrins