Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions

Anal Biochem. 1986 Jul;156(1):220-2. doi: 10.1016/0003-2697(86)90176-4.


Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carbodiimides*
  • Glycine
  • Hemocyanins
  • Solubility
  • Succinimides*
  • Water


  • Carbodiimides
  • Succinimides
  • Water
  • N-hydroxysulfosuccinimide
  • Hemocyanins
  • Glycine