Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum

Methods Mol Biol. 2023:2681:399-406. doi: 10.1007/978-1-0716-3279-6_23.


Deep sequence-coupled biopanning (DSCB) is a powerful tool that couples affinity selection of a bacteriophage MS2 virus-like particle peptide display platform with deep sequencing. While this approach has been used successfully to investigate pathogen-specific antibody responses in human sera, data analysis is time-consuming and complicated. Here, we describe a streamlined data analysis method for DSCB using MATLAB, expanding the potential for this approach to be deployed rapidly and consistently.

Keywords: Affinity selection; Antibodies; Deep sequencing; MATLAB; Pathogens; Phage display; Serum; Virus-like particle.

MeSH terms

  • Antibodies / blood
  • Antibody Formation
  • Bioprospecting / methods
  • Cell Surface Display Techniques / methods
  • High-Throughput Nucleotide Sequencing* / methods
  • Software*


  • Antibodies