Proteomic Changes in Methicillin-Resistant Staphylococcus aureus Exposed to Cannabinoids

J Nat Prod. 2023 Jul 28;86(7):1690-1697. doi: 10.1021/acs.jnatprod.3c00064. Epub 2023 Jul 6.


Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen that causes a wide range of infections. Its resistance to β-lactam antibiotics complicates treatment due to the limited number of antibiotics with activity against MRSA. To investigate development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. In this study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds and analyzed using proteomics to assess the changes in physiology. Subjecting MRSA to nonlethal levels of methicillin resulted in an increased production of penicillin-binding protein 2 (PBP2). Exposure to cannabinoids showed antibiotic activity against MRSA, and differential proteomics revealed reduced levels of proteins involved in the energy production as well as PBP2 when used in combination with methicillin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / metabolism
  • Cannabinoids* / chemistry
  • Cannabinoids* / pharmacology
  • Humans
  • Methicillin / metabolism
  • Methicillin-Resistant Staphylococcus aureus* / drug effects
  • Microbial Sensitivity Tests
  • Penicillin-Binding Proteins / metabolism
  • Proteomics


  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Methicillin
  • Penicillin-Binding Proteins
  • Cannabinoids