Isolation of an amino terminal extended form of basic fibroblast growth factor

Biochem Biophys Res Commun. 1986 Jul 31;138(2):580-8. doi: 10.1016/s0006-291x(86)80536-8.

Abstract

Extraction of bovine pituitaries in the presence of enzyme inhibitors (2 mM PMSF, 2 mM sodium tetrathionate, 15 microM pepstatin A, and 1 mM EDTA) resulted in the isolation of two distinct forms of basic fibroblast growth factor. Partial characterization of both molecules showed one form to be identical to basic FGF(1-146) which has already been reported by our laboratory. The second form was estimated by SDS-PAGE to have a molecular weight of 17,000 Daltons which is slightly larger than that of basic FGF(1-146). Amino acid analysis shows the presence of 8 new residues more than basic FGF(1-146) which accounts for the difference in molecular weight. Gas-phase sequencing of this molecule indicated that it bears a blocked amino terminus. Furthermore, this higher molecular weight form of basic FGF did not show immunoreactivity with antibodies specific for the amino terminus of basic FGF(1-146) but cross reacted with antibodies generated against midportion fragments of basic FGF(1-146), indicating that the molecule is amino terminally extended. Like basic FGF(1-146), the molecule is a potent mitogenic factor for vascular endothelial cells. Taken together these results demonstrate the existence of a precursor form of basic FGF which is extended by 8 residues at the amino terminus with the first residue being blocked.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Antigen-Antibody Complex
  • Cattle
  • Chromatography, High Pressure Liquid
  • Cross Reactions
  • Fibroblast Growth Factors / isolation & purification*
  • Immune Sera
  • Molecular Weight
  • Pituitary Gland / analysis*

Substances

  • Amino Acids
  • Antigen-Antibody Complex
  • Immune Sera
  • Fibroblast Growth Factors