Implemented impediment of extracellular electron transfer-dependent anammox process :Unstable nitrogen removal efficiency and decreased abundance of anammox bacteria

Abstract

The present study investigates the extracellular electron transfer (EET)-dependent anammox process as a promising approach for sustainable wastewater treatment. The study examines the performance and metabolic pathway of the EET-dependent anammox process in comparison to the nitrite-dependent anammox process. The EET-dependent reactor successfully achieved nitrogen removal with a maximum removal efficiency of 93.2%, although it exhibited a lower ability to sustain high nitrogen removal load when compared to the nitrite-dependent anammox process, which poses opportunity and challenge for ammonia-wastewater treatment under applied voltage conditions. Nitrite was identified as a critical factor responsible for the changes in microbial community structure, resulting in a significant reduction in nitrogen removal load in the absence of nitrite. The study further suggests that the Candidatus Kuenenia species could dominate the EET-dependent anammox process, while nitrifying and denitrifying bacteria also contribute to the nitrogen removal in this system.

Keywords: Anammox; Biological nitrogen removal; Extracellular electron transfer; Nitrite.