Isolation of wheat thylakoids for protein analysis

Chao-Yan Chang; Jin-Ying Gou

doi:10.1016/j.mex.2023.102266

Isolation of wheat thylakoids for protein analysis

MethodsX. 2023 Jun 20:11:102266. doi: 10.1016/j.mex.2023.102266. eCollection 2023 Dec.

Authors

Chao-Yan Chang¹, Jin-Ying Gou^{1

2}

Affiliations

¹ School of Life Sciences, Fudan University, Shanghai 200438, China.
² College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.

Abstract

Thylakoids host a large number of proteins to confer photosynthesis and chemical biosynthesis essential for plant survival and growth. Successful isolation of high-quality thylakoids is the first step to studying the compositions and function of thylakoid protein and metabolites. Nevertheless, former studies isolated chloroplasts and thylakoids using a high-speed centrifuge with Percoll, which was expensive and unfriendly to the environment. The method presented here aims to establish a simple and inexpensive method to isolate high-quality thylakoids for protein analysis by utilizing sucrose instead of Percoll to reduce the cost and modify the centrifuge speed into the range usually used in labs.

Keywords: Chloroplast; Thylakoid; Western blot; Wheat; Wheat thylakoids isolation.