RLI2 regulates Arabidopsis female gametophyte and embryo development by facilitating the assembly of the translational machinery

Cell Rep. 2023 Jul 25;42(7):112741. doi: 10.1016/j.celrep.2023.112741. Epub 2023 Jul 6.

Abstract

Eukaryotic protein translation is a complex process that requires the participation of different proteins. Defects in the translational machinery often result in embryonic lethality or severe growth defects. Here, we report that RNase L inhibitor 2/ATP-BINDING CASSETTE E2 (RLI2/ABCE2) regulates translation in Arabidopsis thaliana. Null mutation of rli2 is gametophytic and embryonic lethal, whereas knockdown of RLI2 causes pleiotropic developmental defects. RLI2 interacts with several translation-related factors. Knockdown of RLI2 affects the translational efficiency of a subset of proteins involved in translation regulation and embryo development, indicating that RLI2 has critical roles in these processes. In particular, RLI2 knockdown mutant exhibits decreased expression of genes involved in auxin signaling and female gametophyte and embryo development. Therefore, our results reveal that RLI2 facilitates assembly of the translational machinery and indirectly modulates auxin signaling to regulate plant growth and development.

Keywords: CP: Plants; RLI2; auxin; embryo; female gametophyte; translation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters* / genetics
  • ATP-Binding Cassette Transporters* / metabolism
  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / metabolism
  • Carrier Proteins / metabolism
  • Gene Expression Regulation, Plant
  • Indoleacetic Acids / metabolism
  • Mutation / genetics
  • Ovule / genetics
  • Ovule / metabolism

Substances

  • Arabidopsis Proteins
  • Carrier Proteins
  • Indoleacetic Acids
  • At4g19210 protein, Arabidopsis
  • ATP-Binding Cassette Transporters