Highly sensitive electrochemical peptide-based biosensor for marine biotoxin detection using a bimetallic platinum and ruthenium nanoparticle-tethered metal-organic framework modified electrode

Chikkili Venkateswara Raju; Y Veera Manohara Reddy; Chae Hwan Cho; Hyeon Ho Shin; Tae Jung Park; Jong Pil Park

doi:10.1016/j.foodchem.2023.136811

Highly sensitive electrochemical peptide-based biosensor for marine biotoxin detection using a bimetallic platinum and ruthenium nanoparticle-tethered metal-organic framework modified electrode

Food Chem. 2023 Dec 1:428:136811. doi: 10.1016/j.foodchem.2023.136811. Epub 2023 Jul 4.

Authors

Chikkili Venkateswara Raju¹, Y Veera Manohara Reddy¹, Chae Hwan Cho¹, Hyeon Ho Shin², Tae Jung Park³, Jong Pil Park⁴

Affiliations

¹ Basic Research Laboratory, Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
² Library of Marine Samples, Korea Institute of Ocean Science & Technology, Geoje 53201, Republic of Korea.
³ Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
⁴ Basic Research Laboratory, Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea. Electronic address: jppark@cau.ac.kr.

PMID: 37423105
DOI: 10.1016/j.foodchem.2023.136811

Abstract

Saxitoxin (STX) is a highly toxic small-molecule cyanotoxin that is water-soluble, stable in acidic media, and thermostable. STX is hazardous to human health and the environment in ocean, thus it is an important to detect it at very low concentrations. Herein, we developed an electrochemical peptide-based biosensor for the trace detection of STX in different sample matrix utilizing differential pulse voltammetry (DPV) signal. We synthesized the nanocomposite of zeolitic imidazolate framework-67 (ZIF-67) decorated bimetallic platinum (Pt) and ruthenium (Ru) nanoparticles (Pt-Ru@C/ZIF-67) using impregnation method. The nanocomposite modified with screen-printed electrode (SPE) was subsequently used to detect STX in the range of 1-1,000 ng mL^-1, with a detection limit (LOD) of 26.7 pg mL^-1. The developed peptide-based biosensor is highly selective and sensitive towards STX detection, thus it represents a promising strategy for the development of novel portable bioassay for monitoring various hazardous molecules in aquatic food chains.

Keywords: Cyanotoxin; Metal–organic framework; STX binding peptide; Saxitoxin; Shellfish poisoning.

MeSH terms

Biosensing Techniques* / methods
Electrochemical Techniques / methods
Electrodes
Humans
Limit of Detection
Metal-Organic Frameworks*
Nanoparticles*
Platinum / chemistry
Ruthenium*
Saxitoxin

Substances

Platinum
Metal-Organic Frameworks
Ruthenium
Saxitoxin