Propagation, Culture, and Characterization of Renal Fibroblasts

Tim D Hewitson; Edward R Smith

doi:10.1007/978-1-0716-3179-9_2

Propagation, Culture, and Characterization of Renal Fibroblasts

Methods Mol Biol. 2023:2664:13-29. doi: 10.1007/978-1-0716-3179-9_2.

Authors

Tim D Hewitson^{1

2}, Edward R Smith^{3

4}

Affiliations

¹ Department of Nephrology, The Royal Melbourne Hospital, Parkville, Melbourne, VIC, Australia. tim.hewitson@mh.org.au.
² Department of Medicine, University of Melbourne, Parkville, Melbourne, VIC, Australia. tim.hewitson@mh.org.au.
³ Department of Nephrology, The Royal Melbourne Hospital, Parkville, Melbourne, VIC, Australia.
⁴ Department of Medicine, University of Melbourne, Parkville, Melbourne, VIC, Australia.

PMID: 37423979
DOI: 10.1007/978-1-0716-3179-9_2

Abstract

The renal fibroblast, and phenotypically related myofibroblast, are universally present in all forms of progressive kidney disease. The in vitro study of the fibroblast, its behaviour, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary renal fibroblasts from kidney cortex. Techniques for their isolation, subculture, characterization, and cryogenic storage and retrieval are described in detail.

Keywords: Culture; Explants; Fibroblasts; Kidney; Propagation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fibroblasts
Humans
Kidney Diseases*
Kidney*