Glicentin 1-61 probably represents a major fraction of glucagon-related peptides in plasma of anaesthetized uraemic pigs

Diabetologia. 1986 Jul;29(7):462-7. doi: 10.1007/BF00506540.


Uraemia was induced in pigs by ligation of the renal vascular pedicle, and uraemic plasma was analysed for glucagon and glucagon-related peptides. A preponderance of large molecular weight (Mr) components comprising glicentin and moieties of slightly lower Mr was found, accounting for 73 +/- 3% (mean +/- SEM, n = 12) of the total plasma glucagon-like immunoreactivity. Comparisons with glicentin 1-61, produced by controlled, stepwise, consecutive digestion of purified natural glicentin with carboxypeptidases (carboxypeptidase A followed by carboxypeptidase B, and again by carboxypeptidase A and B), gel filtration, ion exchange chromatography, reverse phase HPLC and radioimmunoassays for the glucagon sequences 6-15 and 19-29 and for the glicentin sequence 12-30 all indicate that glicentin 1-61 constitutes approximately 57% of the large Mr glucagon-related peptides found in uraemia in pigs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography
  • Chromatography, Gel
  • Glucagon / blood
  • Glucagon / immunology*
  • Molecular Weight
  • Peptide Fragments / blood
  • Peptide Fragments / immunology
  • Proglucagon
  • Protein Precursors / blood
  • Protein Precursors / immunology*
  • Radioimmunoassay
  • Swine
  • Uremia / blood*


  • Peptide Fragments
  • Protein Precursors
  • Proglucagon
  • Glucagon