A Comparative Study of Skin Changes in Different Species of Mice in Chronic Photoaging Models

Meifen Lin; Xiaoran Liu; Xueer Wang; Yinyan Chen; Yijia Zhang; Jinfu Xu; Lingwei Bu; Yarui Zhang; Fengting Liang; Xinyue Zhang; Bingli Huang; Min Zhang; Lin Zhang

doi:10.3390/ijms241310812

A Comparative Study of Skin Changes in Different Species of Mice in Chronic Photoaging Models

Int J Mol Sci. 2023 Jun 28;24(13):10812. doi: 10.3390/ijms241310812.

Authors

Affiliations

¹ GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
² Guangzhou Dublin International College of Life Sciences and Technology, South China Agricultural University, Guangzhou 510642, China.
³ School of Public Health, Southern Medical University, Guangzhou 510515, China.

Abstract

This study aimed to design a novel mouse model of chronic photoaging. We used three different species of mice (C57BL/6J, ICR, and KM) to create a chronic photoaging model of the skin. The irradiation time was gradually increased for 40 consecutive days. The skins of the mice were removed on day 41 and subjected to staining to observe them for morphological changes. Immunohistochemistry was used to detect tumor necrosis factor-α (TNF-α) and p53 expression; superoxide dismutase (SOD) and malondialdehyde (MDA) were measured as well. Compared with C57BL/J mice, which showed hyperpigmentation, the irradiated skin of ICR and KM mice showed more obvious skin thickening and photoaging changes of the collagen and elastic fibers. KM mice had higher levels of inflammation, oxidative stress, and senescent cells. Compared with the 5-month-old KM mice, the photoaging changes of the 9-month-old KM mice were more pronounced, the SOD values were lower, and the MDA values were higher. In summary, KM mice have higher levels of abnormal elastic fibers, inflammation, cellular senescence, and oxidative stress than ICR mice, and are more suitable for studies related to chronic skin photoaging. C57BL/6J mice were found to be suitable for studies related to skin pigmentation due to photoaging.

Keywords: C57BL/6J mice; chronic skin photoaging model; malondialdehyde; superoxide dismutase; ultraviolet.

MeSH terms

Animals
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Skin / metabolism
Skin Aging*
Superoxide Dismutase / metabolism
Ultraviolet Rays / adverse effects

Substances

Superoxide Dismutase

Supplementary concepts

Kunming mice

Grants and funding

This research was funded by the National Natural Science Foundation of China, grant numbers 81402613 and 82073417; the GDMPA Key Laboratory Project of Scientific and Technological Innovation, grant number 2023ZDZ12; the Guangdong Basic and Applied Basic Research Foundation, grant number 2019A1515011213, 2020A1515011067, and 2022A1515010768; the Xinjiang Uygur Autonomous Region Natural Science Foundation, grant number 2019D01A83; and the Medical Scientific Research Foundation of Guangdong Province, grant number A2020136.