Evaluation of the Illumina iSeq whole genome sequencing system for enteric disease surveillance and outbreak detection

Eija Trees; Angela Poates; Ashley Sabol; Patricia LaFon; Jenny Truong; Rebecca Lindsey

doi:10.1016/j.mimet.2023.106784

Evaluation of the Illumina iSeq whole genome sequencing system for enteric disease surveillance and outbreak detection

J Microbiol Methods. 2023 Aug:211:106784. doi: 10.1016/j.mimet.2023.106784. Epub 2023 Jul 13.

Authors

Eija Trees¹, Angela Poates², Ashley Sabol², Patricia LaFon², Jenny Truong², Rebecca Lindsey²

Affiliations

¹ Centers for Disease Control and Prevention, 1600 Clifton Road, Mail stop H23-7, Atlanta, GA 30329, USA. Electronic address: EHyytia-Trees@cdc.gov.
² Centers for Disease Control and Prevention, 1600 Clifton Road, Mail stop H23-7, Atlanta, GA 30329, USA.

PMID: 37451348
DOI: 10.1016/j.mimet.2023.106784

Abstract

The Illumina iSeq low-capacity sequencing platform was evaluated for use in foodborne disease surveillance and outbreak detection. The platform produced high quality sequence data comparable to that of the Illumina MiSeq and was cost-effective with fast turn-around time in low sample volume environments.

Keywords: Allele; Coverage; MiSeq; WGS; iSeq.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Data Accuracy
Disease Outbreaks*
Foodborne Diseases* / epidemiology
Genome, Bacterial
High-Throughput Nucleotide Sequencing
Humans
Whole Genome Sequencing