Expression profile of LncRNA ANRIL, miR-186, miR-181a, and MTMR-3 in patients with preeclampsia

Shymaa E Ayoub; Olfat G Shaker; Rehab Abdelhamid Aboshama; Mohamed K Etman; Abeer A Khalefa; Mohamed M Khamiss Abd Elguaad; Othman M Zaki; Doaa Y Ali; Nada F Hemeda; Amal Amin; Marwa A Ali

doi:10.1016/j.ncrna.2023.06.001

Expression profile of LncRNA ANRIL, miR-186, miR-181a, and MTMR-3 in patients with preeclampsia

Noncoding RNA Res. 2023 Jun 7;8(4):481-486. doi: 10.1016/j.ncrna.2023.06.001. eCollection 2023 Dec.

Authors

Affiliations

¹ Department of Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
² Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
³ Department of Obstetrics and Gynecology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
⁴ Department of Physiology, Faculty of Medicine, Zagazig University, El Zagazig, Egypt.
⁵ Department of Physiology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
⁶ Department of Clinical Pathology, Faculty of Medicine, Damietta University, Damietta, Egypt.
⁷ Department of Clinical and Chemical Pathology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.
⁸ Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
⁹ Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Al Fayoum, Egypt.

Abstract

Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Several studies demonstrated the role of lncRNAs and miRNAs in the pathogenesis of preeclampsia; the aim was to detect the expression profiles of serum LncRNA ANRIL, miR-186, miR-181a, and MTMR-3 in patients with preeclampsia. The study included 160 subjects divided into 80 subjects considered as a control group, 80 patients with preeclampsia. We found that there was a significant difference between the preeclampsia and control groups with up-regulation of miR-186 median (IQR) = 4, 29 (1.35-7.73) (P < 0.0001), miR-181a median (IQR) = 2.45 (0.83-6.52) (P = 0.028), and downregulation of lncRNA ANRIL median (IQR) = 0.35(0.28-0.528) (P < 0.0001), MTMR median (IQR) = 0.32(0.155-1.11), (P < 0.0001). ROC curve of lncRNA ANRIL, miR-186, miR-181a, and MTMR-3 in preeclampsia patients showing the roles of these markers in the diagnosis of preeclampsia. In conclusion, serum LncRNA ANRIL, miR-186, miR-181a, and MTMR-3 could be promising biomarkers in the diagnosis of preeclampsia.

Keywords: LncRNA ANRIL; MTMR-3; Preeclampsia; miR-181a; miR-186.