Mechanism of primer synthesis by Primase-Polymerases

Katerina Zabrady; Arthur W H Li; Aidan J Doherty

doi:10.1016/j.sbi.2023.102652

Mechanism of primer synthesis by Primase-Polymerases

Curr Opin Struct Biol. 2023 Oct:82:102652. doi: 10.1016/j.sbi.2023.102652. Epub 2023 Jul 15.

Authors

Katerina Zabrady¹, Arthur W H Li², Aidan J Doherty³

Affiliations

¹ Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK. Electronic address: https://twitter.com/@KZabrady.
² Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK.
³ Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK. Electronic address: ajd21@sussex.ac.uk.

PMID: 37459807
DOI: 10.1016/j.sbi.2023.102652

Abstract

Members of the primase-polymerase (Prim-Pol) superfamily are found in all domains of life and play diverse roles in genome stability, including primer synthesis during DNA replication, lesion repair and damage tolerance. This review focuses primarily on Prim-Pol members capable of de novo primer synthesis that have experimentally derived structural models available. We discuss the mechanism of DNA primer synthesis initiation by Prim-Pol catalytic domains, based on recent structural and functional studies. We also describe a general model for primer initiation that also includes the ancillary domains/subunits, which stimulate the initiation of primer synthesis.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Catalytic Domain
DNA Primase* / chemistry
DNA Replication*

Substances

DNA Primase

Abstract

Publication types

MeSH terms

Substances

Grants and funding