Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells

Leqian Yu; Emily Ballard; Carlos A Pinzon Arteaga; Jun Wu

doi:10.1007/978-1-0716-3259-8_16

Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells

Methods Mol Biol. 2023:2677:269-280. doi: 10.1007/978-1-0716-3259-8_16.

Authors

Leqian Yu¹, Emily Ballard¹, Carlos A Pinzon Arteaga¹, Jun Wu^{2

3

4}

Affiliations

¹ Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
² Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. jun2.wu@utsouthwestern.edu.
³ Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. jun2.wu@utsouthwestern.edu.
⁴ Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA. jun2.wu@utsouthwestern.edu.

Abstract

Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of the pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.

Keywords: Formative state; Human pluripotent stem cells; Intermediate state; Mouse pluripotent stem cells; Primordial germ cell.

Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells

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