Exposure to Low Concentrations of AMPA Influences Morphology and Decreases Survival During Larval Development in a Widespread Amphibian Species

Abstract

Glyphosate's primary metabolite, AMPA (aminomethylphosphonic acid), is one of the most widely detected anthropogenic substance in surface waters worldwide. However, ecotoxicological studies on the potential effects of this metabolite at environmental concentrations on wildlife are scarce. Yet, due to its chemical properties, AMPA is likely to affect non-target species. In this study, we investigated sublethal effects of environmental concentrations of AMPA on the larval development of a widespread amphibian species, the spined toad Bufo spinosus. We performed a factorial experiment to study the effect of concentration and the timing of exposure (during embryonic development, larval development or both) to AMPA on the morphology, rate of development and survival of tadpoles. AMPA and timing of exposure interactively affected tadpole size (individuals exposed to AMPA after hatching were transitorily smaller, while individuals exposed to AMPA before hatching were longer), but not duration of development. Most of these effects were linked to exposure during embryonic development. Such effects in individuals exposed during embryonic development solely were long-lasting and persisted until the latest larval stages. Finally, we found that exposure to AMPA after hatching (during the larval stage) increased mortality. Exposure to low environmental concentrations of AMPA could have long-lasting consequences on fitness and population persistence. These findings are especially important to take into account at a time when multiple threats can interact to affect wildlife.