miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10

Peng Gao; Haopeng Wang; Huaixu Li; Lei Shu; Zhenyu Han; Shiting Li; Hongwei Cheng; Xingliang Dai

doi:10.7150/jca.84030

miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10

J Cancer. 2023 Jun 19;14(10):1781-1793. doi: 10.7150/jca.84030. eCollection 2023.

Authors

Peng Gao^{1

2}, Haopeng Wang³, Huaixu Li¹, Lei Shu⁴, Zhenyu Han⁵, Shiting Li³, Hongwei Cheng¹, Xingliang Dai¹

Affiliations

¹ Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, China.
² Department of Neurosurgery, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu, China.
³ Department of Neurosurgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China.
⁴ Department of Clinical Medicine, The First Clinical College of Anhui Medical University, Hefei, 230032, Anhui, China.
⁵ Department of Medical Imaging Technology, The First Clinical College of Anhui Medical University, Hefei, 230032, Anhui, China.

Abstract

S100A10, a member of the S100 protein family, is upregulated in multiple human malignancies and plays a key role in regulating tumor progression. This study aimed to reveal the underlying mechanism by which S100A10 in regulates the proliferation, migration, and invasion of glioma. The expression and clinical information data of S100A10 were downloaded from public databases (TCGA, CGGA, and GEPIA2). S100A10 expression levels in glioma tumor tissues and adjacent nontumor tissues were compared by immunohistochemistry (IHC). The functional roles of S100A10 in glioma were assessed by cell counting kit-8 (CCK-8) cell proliferation assay, wound healing assay, transwell assay, and flow cytometry. miRDB and double luciferase assay were used to predict and identify potential S100A10 mRNA-complementary miRNAs, and the roles of miR-21-5p in glioma cell were examined by targeted knockdown or overexpression miR-21-5p in glioma cell lines. We found that S100A10 was overexpressed in glioma tissues and predicted a worse prognosis. S100A10 knockdown significantly inhibited glioma cell proliferation, invasion, and migration. Furthermore, we demonstrated that miR-21-5p inhibits glioma proliferation, migration, and invasion by targeting S100A10. This study showed S100A10 was a new prognostic predictor among glioma patients and provided new insights into the pathogenesis of gliomas, suggesting that miR-21-5p /S100A10 axis may serve as a valuable therapeutic target for glioma.

Keywords: S100A10; glioma; miR-21-5p; prognosis.; progression.