Antennae-enriched expression of candidate odorant degrading enzyme genes in the turnip aphid, Lipaphis erysimi

Chaozhi Shangguan; Yinhui Kuang; Liwei Gao; Bo Zhu; Xue Dong Chen; Xiudao Yu

doi:10.3389/fphys.2023.1228570

Antennae-enriched expression of candidate odorant degrading enzyme genes in the turnip aphid, Lipaphis erysimi

Front Physiol. 2023 Jul 5:14:1228570. doi: 10.3389/fphys.2023.1228570. eCollection 2023.

Authors

Chaozhi Shangguan¹, Yinhui Kuang¹, Liwei Gao¹, Bo Zhu¹, Xue Dong Chen², Xiudao Yu¹

Affiliations

¹ Ganzhou Key Laboratory of Nanling Insect Biology/National Navel Orange Engineering Research Center, College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi, China.
² Entomology and Nematology Department, University of Florida, Gainesville, FL, United States.

Abstract

Aphids heavily rely on their olfactory system for foraging behavior. Odorant-degrading enzymes (ODEs) are essential in preserving the olfactory acuity of aphids by removing redundant odorants in the antennae. Certain enzymes within this group stand out as being enriched and/or biased expressed in the antennae, such as carboxylesterases (CXEs), cytochrome P450 (CYPs), glutathione S-transferases (GSTs), and UDP-glycosyltransferases (UGTs). Here, we performed a comparative transcriptome analysis of antennae and body tissue to isolate the antennal ODE genes of turnip aphid Lipaphis erysimi. A dataset of one CXE, seven CYPs, two GSTs, and five UGTs enriched in the antennae was identified and subjected to sequence analysis. Furthermore, qRT-PCR analyses showed that 13 ODE genes (LeCXE6, LeCYP4c1, LeCYP6a2, LeCYP6a13, LeCYP6a14.2, LeCYP6k1, LeCYP18a1, LeGST1, LeUGT1-7, LeUGT2B7, LeUGT2B13, LeUGT2C1.1, and LeUGT2C1.2) were specifically or significantly elevated in antennal tissues. Among these antennae-enriched ODEs, LeCYP4c1, LeCYP6a2, LeCYP6a13, LeCYP6a14.2, LeCYP18a1, LeUGT2B7, and LeUGT2B13 were found to exhibit significantly higher expression levels in alate aphids compared to apterous and nymph aphids, suggesting their putative role in detecting new host plant location. The results presented in this study highlight the identification and expression of ODE genes in L. erysimi, paving the path to investigate their functional role in odorant degradation during the olfactory processes.

Keywords: antennae-enriched; comparative transcriptome analysis; gene expression; odorant degrading enzyme; turnip aphid.

Grants and funding

This work is funded by the Natural Science Foundation for Distinguished Young Scholars of Jiangxi province (grant no. 20212ACB215001), the Double Thousand Plan of Jiangxi Province (grant no. jxsq2019101058), and the Natural Science Foundation of Jiangxi Province (20212BAB215003).