Cell-surface biotinylation of GABAA receptors in mouse hippocampal slices after sevoflurane anesthesia

Abstract

Here, we present a protocol for studying the cell-surface proteins in hippocampal slices after in vivo administration of sevoflurane, an inhaled general anesthetic drug, to mice. We describe steps for anesthetic delivery, hippocampal slice preparation, and cell-surface biotinylation. We then detail the isolation of surface proteins and their quantification through Western blotting. This protocol can be adapted to study changes in other surface proteins following exposure to various general anesthetic drugs. For complete details on the use and execution of this protocol, please refer to Wang et al. (2012),¹ Zurek et al. (2014),² and Yu et al. (2019).³.

Keywords: Cell Membrane; Molecular Biology; Neuroscience; Protein Expression and Purification.