Clinical Cytometry for Platelets and Platelet Disorders

Andrew L Frelinger 3rd; Benjamin E J Spurgeon

doi:10.1016/j.cll.2023.04.008

Clinical Cytometry for Platelets and Platelet Disorders

Clin Lab Med. 2023 Sep;43(3):445-454. doi: 10.1016/j.cll.2023.04.008. Epub 2023 May 29.

Authors

Andrew L Frelinger 3rd¹, Benjamin E J Spurgeon²

Affiliations

¹ Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA 02115, USA. Electronic address: andrew.frelinger@childrens.harvard.edu.
² Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA 02115, USA.

PMID: 37481322
DOI: 10.1016/j.cll.2023.04.008

Abstract

Clinical flow cytometry tests for inherited and acquired platelet disorders are useful diagnostic tools but are not widely available. Flow cytometric methods are available to detect inherited glycoprotein deficiencies, granule release (secretion defects), drug-induced thrombocytopenias, presence of antiplatelet antibodies, and pharmacodynamic inhibition by antiplatelet agents. New tests take advantage of advanced multicolor cytometers and allow identification of novel platelet subsets by high-dimensional immunophenotyping. Studies are needed to evaluate the value of these new tests for diagnosis and monitoring of therapy in patients with platelet disorders.

Keywords: Blood platelet disorders; Flow cytometry; Phenotyping; Platelet activation; Platelet function tests; Thrombocytopenia.

Publication types

Review

MeSH terms

Antibodies
Blood Platelet Disorders* / diagnosis
Blood Platelets* / physiology
Flow Cytometry / methods
Humans
Immunophenotyping

Substances

Antibodies