GPD1 inhibits the carcinogenesis of breast cancer through increasing PI3K/AKT-mediated lipid metabolism signaling pathway

Zhengchao Xia; Ningming Zhao; Mingzhou Liu; DanDan Jiang; Shanjun Gao; Peizhi Ma; Li Huang

doi:10.1016/j.heliyon.2023.e18128

GPD1 inhibits the carcinogenesis of breast cancer through increasing PI3K/AKT-mediated lipid metabolism signaling pathway

Heliyon. 2023 Jul 8;9(7):e18128. doi: 10.1016/j.heliyon.2023.e18128. eCollection 2023 Jul.

Authors

Zhengchao Xia¹, Ningming Zhao¹, Mingzhou Liu¹, DanDan Jiang¹, Shanjun Gao², Peizhi Ma¹, Li Huang³

Affiliations

¹ Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, China.
² Microbiome Laboratory, Henan Provincial People's Hospital, Zhengzhou, Henan, China.
³ Department of Pathology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, China.

Abstract

Glycerol 3-phosphate dehydrogenase 1 (GPD1) acts as a tumor suppressor in various types of cancer. However, the mechanisms of GPD1 anti-tumor remain unclear in breast cancer. This study aims to explore the function and clinical relevance of GPD1 in breast cancer. We confirmed that GPD1 inhibited the ability of proliferation, migration, and invasion in GPD1 overexpression breast cancer cells by CCK-8, wound healing, and Transwell assays, respectively. We found that GPD1 overexpression activated the lipid synthesis pathway and PI3K/AKT signaling pathway. The inhibitory effect of GPD1 on breast cancer cells was also weakened after treatment with LY294002, a PI3K/AKT pathway inhibitor. These results indicated that GPD1 suppressed the carcinogenesis of breast cancer through increasing PI3K/AKT-mediated lipid signaling pathways. Meanwhile, we detected that the relationship between GPD1 level and survival rate presents a positive correlation in breast cancer patients from the Cancer Genome Atlas (TCGA) database. Therefore, GPD1 can be a prognostic biomarker and target in developing therapeutic strategies for breast cancer patients.

Keywords: Biomarker; Breast cancer; Glycerol 3-phosphate dehydrogenase 1; Lipid metabolism; PI3K/AKT signaling pathway.