Essential oil-grafted copper nanoparticles as a potential next-generation fungicide for holistic disease management in maize

Lham Dorjee; Robin Gogoi; Deeba Kamil; Rajesh Kumar; Tapan Kumar Mondal; Sudeepta Pattanayak; Bishal Gurung

doi:10.3389/fmicb.2023.1204512

Essential oil-grafted copper nanoparticles as a potential next-generation fungicide for holistic disease management in maize

Front Microbiol. 2023 Jul 6:14:1204512. doi: 10.3389/fmicb.2023.1204512. eCollection 2023.

Authors

Lham Dorjee¹, Robin Gogoi¹, Deeba Kamil¹, Rajesh Kumar², Tapan Kumar Mondal³, Sudeepta Pattanayak¹, Bishal Gurung⁴

Affiliations

¹ Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
² Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, India.
³ Division of Molecular Biology and Biotechnology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
⁴ Division of Forecasting and Agricultural Systems Modelling, ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India.

Abstract

Sustainable food production is necessary to meet the demand of the incessantly growing human population. Phytopathogens pose a major constraint in food production, and the use of conventional fungicides to manage them is under the purview of criticism due to their numerous setbacks. In the present study, essential oil-grafted copper nanoparticles (EGC) were generated, characterized, and evaluated against the maize fungal pathogens, viz., Bipolaris maydis, Rhizoctonia solani f. sp. sasakii, Macrophomina phaseolina, Fusarium verticillioides, and Sclerotium rolfsii. The ED₅₀ for the fungi under study ranged from 43 to 56 μg ml^-1, and a significant inhibition was observed at a low dose of 20 μg ml^-1 under in vitro conditions. Under net house conditions, seed treatment + foliar spray at 250 and 500 mg L^-1 of EGC performed remarkably against maydis leaf blight (MLB), with reduced percent disease index (PDI) by 27.116 and 25.292%, respectively, in two Kharif seasons (May-Sep, 2021, 2022). The activity of enzymatic antioxidants, viz., β-1, 3-glucanase, PAL, POX, and PPO, and a non-enzymatic antioxidant (total phenolics) was increased in treated maize plants, indicating host defense was triggered. The optimum concentrations of EGC (250 mg L^-1 and 500 mg L^-1) exhibited improved physiological characteristics such as photosynthetic activity, shoot biomass, plant height, germination percentage, vigor index, and root system traits. However, higher concentrations of 1,000 mg L^-1 rendered phytotoxicity, reducing growth, biomass, and copper bioaccumulation to high toxic levels, mainly in the foliar-sprayed maize leaves. In addition, EGC and copper nanoparticles (CuNPs) at 1,000 mg L^-1 reduced the absorption and concentration of manganese and zinc indicating a negative correlation between Cu and Mn/Zn. Our study proposes that the CuNPs combined with EO (Clove oil) exhibit astounding synergistic efficacy against maize fungal pathogens and optimized concentrations can be used as an alternative to commercial fungicides without any serious impact on environmental health.

Keywords: MLB disease; essential oil-grafted CuNPs; maize; management; physiological traits; phytopathogens.