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. 2023 Jul 11;13(7):840.
doi: 10.3390/metabo13070840.

The Implications in Meat Quality and Nutrition by Comparing the Metabolites of Pectoral Muscle between Adult Indigenous Chickens and Commercial Laying Hens

Lingqian Yin  1 , Li Liu  1 , Yuan Tang  1 , Qian Chen  1 , Donghao Zhang  1 , Zhongzhen Lin  1 , Yan Wang  1 , Yiping Liu  1
Affiliations

The Implications in Meat Quality and Nutrition by Comparing the Metabolites of Pectoral Muscle between Adult Indigenous Chickens and Commercial Laying Hens

Lingqian Yin et al. Metabolites. .

Abstract

Aged chickens are often a secondary dietary choice, owing to the poor organoleptic qualities of their meat. With regard to the meat quality of chickens, the metabolic profiles of pectoral muscle in Guangyuan grey chickens (group G) and Hy-Line grey hens (group H) aged 55 weeks were compared via ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A total of 74 metabolites were identified with differential changes in the ion model. Lipids and lipid-like molecules comprised the largest proportion among the different metabolites. The content of myristic acid and palmitic acid were found to be higher in the pectoral muscle of group G, while group H showed significantly higher levels of glycerophospholipid molecules, such as LPC(18:2/0:0), Pi(38:5), Pc(16:0/16:0), and Pe(16:1e/14-hdohe). KEGG pathway analysis indicated that the abundant metabolites in group G were mainly involved in energy metabolism and fatty acid biosynthesis and metabolism, whereas those of group H were mainly attributed to the metabolism of unsaturated fatty acids and amino acids. Overall, the differences in lipid and amino acid metabolism in pectoral muscle appear to be responsible for the difference in meat quality between indigenous chickens and commercial laying hens.

Keywords: UHPLC-MS/MS; chicken; lipid metabolism; non-targeted metabonomics; pectoral muscle.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The different metabolite analysis between groups. (A) Volcanic plot of metabolites in the positive ion modes. (B) Volcanic plot of metabolites in the negative ion modes. Red circle, upregulated significantly differential metabolites; blue circle, down-regulated significantly differential metabolites. (C) PCA scores of group H and G. (D) OPLS-DA scores of group H and G.
Figure 2
Figure 2
Heat maps of correlation between differential metabolites in the positive (A) and negative (B) ion modes. Red, positive correlation; blue, negative correlation.
Figure 2
Figure 2
Heat maps of correlation between differential metabolites in the positive (A) and negative (B) ion modes. Red, positive correlation; blue, negative correlation.
Figure 3
Figure 3
Oil red O staining of pectoral muscle sections of Guangyuan grey chicken (A) and Hy-Line grey hen (B). The sample was observed with an optical microscope (100×). Red arrows point to lipid droplets.
Figure 4
Figure 4
The content of triglyceride in group G and H.
See this image and copyright information in PMC

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