GABA content and an antioxidant profile positively correlated with the anticonvulsive activity of Microcos paniculata in acute seizure mice

S M Kamruzzaman; Latifa Bulbul; Md Zahir Alam; Md Mostafizur Rahman

doi:10.1016/j.heliyon.2023.e18295

GABA content and an antioxidant profile positively correlated with the anticonvulsive activity of Microcos paniculata in acute seizure mice

Heliyon. 2023 Jul 14;9(7):e18295. doi: 10.1016/j.heliyon.2023.e18295. eCollection 2023 Jul.

Authors

S M Kamruzzaman¹, Latifa Bulbul², Md Zahir Alam², Md Mostafizur Rahman³

Affiliations

¹ Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
² Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh.
³ Department of Chemistry, National University, Dhaka, Bangladesh.

Abstract

This study evaluated the effects of different parts of M. paniculata (MP) extracts on convulsions and antioxidant activities in mice. Six polyphenolic compounds were identified, where epicatechin and quercetin have been identified in the highest amounts (23.01 and 32.23 mg/100 g of dry MP extract, respectively) in MP leaf and stem extracts, using Ultra Performance Liquid Chromatography. 7-day oral administration of MP at doses of 100, 200, and 400 mg/kg body weight (BW) significantly reduced convulsions and reduced mortality rates compared with seizure inducer groups. Antioxidant potentials were measured by superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH) content in whole-brain homogenates. Gamma-aminobutyric acid (GABA) levels significantly increased in leaves and stem-treated groups, suggesting that MP leaves and stems have potent antioxidant properties that can attenuate convulsions by modulating the GABAergic system and antioxidant activities.

Keywords: Antioxidant enzymes; Epilepsy; GABA; Lipid peroxidation; Plant extracts.