Bacterial, short-chain fatty acid and gas profiles of partially hydrolyzed guar gum in vitro fermentation by human fecal microbiota

Xiong-E Pi; Hao Fu; Xiao-Xia Yang; Zai-Chun Yu; Wei-Lin Teng; Yinjun Zhang; Xue-Wei Ye; Hui Hui Quan; Li-Zhi Lu; Wei Liu

doi:10.1016/j.foodchem.2023.137006

Bacterial, short-chain fatty acid and gas profiles of partially hydrolyzed guar gum in vitro fermentation by human fecal microbiota

Food Chem. 2024 Jan 1:430:137006. doi: 10.1016/j.foodchem.2023.137006. Epub 2023 Jul 27.

Authors

Xiong-E Pi¹, Hao Fu², Xiao-Xia Yang³, Zai-Chun Yu³, Wei-Lin Teng⁴, Yinjun Zhang⁵, Xue-Wei Ye⁶, Hui Hui Quan⁷, Li-Zhi Lu⁸, Wei Liu⁹

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China. Electronic address: pixe@zaas.ac.cn.
² State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China.
³ College of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
⁴ Department of Infectious Disease Control and Prevention, HangZhou Center for Disease Control and Prevention, Hangzhou 310006, China.
⁵ College of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address: zhangyj@zjut.edu.cn.
⁶ Shulan International Medical College, Department of Basic Medical Sciences, Zhejiang Shuren University, Hangzhou 310015, China. Electronic address: 601215@zjsru.edu.cn.
⁷ State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China. Electronic address: quanhh@zaas.ac.cn.
⁸ State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China. Electronic address: lulz@zaas.ac.cn.
⁹ State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, China. Electronic address: liuw@zaas.ac.cn.

PMID: 37541036
DOI: 10.1016/j.foodchem.2023.137006

Abstract

Carbohydrates with different structures have metabolic differences in the human body, as well as individual differences. The present study aimed to investigate the effects of bacterial, short-chain fatty acids (SCFAs) and gas profiles of partially hydrolyzed guar gum (PHGG) on the fecal microbiota of 41 Chinese individuals by simulated fermentation in vitro. Results showed that PHGG stimulated the growth of Bifidobacterium and Faecalibacterium, inhibited the growth of Escherichia-Shigella, Klebsiella, and Dorea, and induced the production of fermentation gases (CO2, and H2) and SCFAs (acetic acid, butyric acid). Furthermore, Bifidobacterium was significantly increased in the young female and the old male-originated samples, while Klebsiella was significantly decreased in the old female ones after PHGG intervention, and there were also certain differences in gases and SCFAs among different population samples. These findings indicate that PHGG can modulate gut microbiota and metabolism well, whereas its use varies in different populations.

Keywords: Gas production; Gut microbiota; Partially hydrolyzed guar gum; SCFAs.

MeSH terms

Bacteria / metabolism
Dietary Fiber* / analysis
Fatty Acids, Volatile / metabolism
Feces / microbiology
Female
Fermentation
Galactans / chemistry
Gases
Gastrointestinal Microbiome*
Humans
Male
Mannans / chemistry
Plant Gums / chemistry

Substances

guar gum
Dietary Fiber
Fatty Acids, Volatile
Galactans
Mannans
Plant Gums
Gases