Drug Resistance Profile Among HIV-1 Infections Experiencing ART with Low-Level Viral Load in Guangdong China During 2011-2022: A Retrospective Study

Yun Lan; Xuemei Ling; Xizi Deng; Yaqing Lin; Junbin Li; Liya Li; Ruiying He; Weiping Cai; Feng Li; Linghua Li; Fengyu Hu

doi:10.2147/IDR.S419610

Drug Resistance Profile Among HIV-1 Infections Experiencing ART with Low-Level Viral Load in Guangdong China During 2011-2022: A Retrospective Study

Infect Drug Resist. 2023 Jul 31:16:4953-4964. doi: 10.2147/IDR.S419610. eCollection 2023.

Authors

Yun Lan^#¹, Xuemei Ling^#^{1

2}, Xizi Deng^#¹, Yaqing Lin¹, Junbin Li^{1

2}, Liya Li¹, Ruiying He¹, Weiping Cai¹, Feng Li^#¹, Linghua Li¹, Fengyu Hu¹

Affiliations

¹ Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, 510440, People's Republic of China.
² Guangdong Center for Diagnosis and Treatment of AIDS, Guangzhou, 510060, People's Republic of China.

^# Contributed equally.

Abstract

Background: Antiretroviral therapy (ART) efficiently reduces the morbidities and mortalities caused by HIV-1 infection and prevents the HIV epidemic. However, virologic failure (VF) occurs in some patients receiving ART experience, especially increases in those patients with intermittent or persistent low-level viremia (LLV). The presence of drug resistance mutations (DRMs) in LLV was a strong predictor of subsequent VF. The data on drug resistance (DR) or DRMs for HIV-1 infections at low-level viral load (LLVL) are limited in China.

Objective: To monitor the prevalence of HIV-1 drug resistance and to evaluate the risk factors associated with drug resistance in LLVL HIV-1 infections during ART in Guangdong, China.

Methods: Plasma samples with LLVL during ART in Guangdong Province between Jan 2011 and Dec 2022 were subjected to a modified reverse-transcription PCR with a pre-step of virus concentration by ultracentrifugation before extraction and the Sanger sequencing. Then, the genotypic resistance test was performed and DR was analyzed by the Stanford HIVDB program. Finally, DR-associated factors were identified by logistic regression analysis.

Results: We found that CRF01_AE (53.57%) and CRF07_BC (25.07%) were the dominant HIV-1 genotypes in LLVL in Guangdong between 2011 and 2022 but that the percentage of CRF01_AE showed a trend of decrease over time. M46 (1.49%), M184 (30.91%), and K103 (21.46%) were the dominant PI-, NRTI-, and NNRTI-associated mutations, respectively. The total DR rate was 47.06%. Specifically, PI (3.71%) showed a significantly lower DR rate than NNRTI (40.74%) and NRTI (34.14%). Duration of ART, initial ART regimen, ethnicity, and WHO clinical stages were associated with DR.

Conclusion: The drug resistance rate among the LLVL during ART in Guangdong, China is high. The risk factors associated with HIV drug resistance should be seriously considered for better control.

Keywords: HIV; drug resistance; drug resistance mutation; low-level viral load.

Grants and funding

Our study was supported by the Guangzhou Science and Technology Plan Project (202201011646, 202201020503) The funders had no role in study design, data collection, analysis, the decision to publish, and preparation of the article.