A simple PCR assay for the identification of the novel Streptococcus pneumoniae serotype 7D

J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001739.


The identification of the novel pneumococcal serotype 7D by Neufeld quellung reaction requires significant expertise. To circumvent this, we developed a simple serotype-specific PCR method to discriminate serotype 7D from the closely related serotypes 7C, 7B and 40. The established PCR was validated with the strain collection of the German National Reference Center for Streptococci (GNRCS). However, no isolate initially assigned as serotype 7B, 7C or 40 was identified as serotype 7D.

Keywords: 7D; PCR; Streptococcus pneumoniae; hybrid cps; serotype.

MeSH terms

  • Humans
  • Pneumococcal Infections* / diagnosis
  • Pneumococcal Vaccines
  • Polymerase Chain Reaction
  • Serogroup
  • Serotyping
  • Streptococcus pneumoniae*


  • Pneumococcal Vaccines