Eighty strains of Yersinia enterocolitica were examined for their ability to invade HeLa cells. The following O-serotypes were found to be invasive: 1, 3, 4a, 4c, 5b, 8, 9, 15a, 18, 20, 21 and 22. Biochemically, these types are either indole-, aesculin-, and salicin-negative, or indole-positive but aesculin- and salicin-negative. Other serotypes of Y. enterocolitica did not invade HeLa cells. Biochemically, these non-invasive strains are indole-, aesculin- and salicin-positive. Also the non-sucrose-fermenting strains of O-serotypes 11 and 12 were non-invasive. The interaction with HeLa cells was not restricted to viable bacteria, since also bacterial cells inactivated by formalin or ultraviolet light could gain entrance to the HeLa cells. Heating of the bacteria to 100 degrees C for 30 min. completely inhibited their invasiveness. Monolayer cultures of porcine kidney cells were also invaded by strains which invaded HeLa cells.