Phytochemical Analysis and In Vitro Antileukemic Activity of Alkaloid-Enriched Extracts from Vinca sardoa (Stearn) Pignatti

Daniela De Vita; Claudio Frezza; Fabio Sciubba; Chiara Toniolo; Camilla Badiali; Rita Petrucci; Martina Bortolami; Paola Di Matteo; Daniele Rocco; Annarita Stringaro; Marisa Colone; Andrea Maxia; Maria Teresa Petrucci; Mauro Serafini; Sebastiano Foddai

doi:10.3390/molecules28155639

Phytochemical Analysis and In Vitro Antileukemic Activity of Alkaloid-Enriched Extracts from Vinca sardoa (Stearn) Pignatti

Molecules. 2023 Jul 25;28(15):5639. doi: 10.3390/molecules28155639.

Authors

Affiliations

¹ Department of Environmental Biology, "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
² NMR-Based Metabolomics Laboratory, "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
³ Department of Basic and Applied Sciences for Engineering, "Sapienza" University of Rome, Via Castro Laurenziano 7, 00161 Rome, Italy.
⁴ National Center for Drug Research and Evaluation, Italian National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy.
⁵ Laboratory of Economic and Pharmaceutical Botany, Department of Life and Environmental Sciences, University of Cagliari, Viale S. Ignazio da Laconi 13, 09123 Cagliari, Italy.
⁶ Department of Cellular Biotechnologies and Hematology, "Sapienza" University of Rome, Via Benevento 6, 00161 Rome, Italy.

Abstract

Vinca sardoa (Stearn) Pignatti, known as Sardinian periwinkle, is widely diffused in Sardinia (Italy). This species contains indole alkaloids, which are known to have a great variety of biological activities. This study investigated the antileukemic activity against a B lymphoblast cell line (SUP-B15) of V. sardoa alkaloid-rich extracts obtained from plants grown in Italy, in Iglesias (Sardinia) and Rome (Latium). All the extracts showed a good capacity to induce reductions in cell proliferation of up to 50% at the tested concentrations (1-15 µg/mL). Moreover, none of the extracts showed cytotoxicity on normal cells at all the studied concentrations.

Keywords: ESI-MS/MS characterization; HPLC-PDA-ESI-MS analysis; Sardinian periwinkle; Vinca sardoa (Stearn) Pignatti; Vinca difformis subsp. sardoa Stearn; alkaloids; antileukemic activity.

MeSH terms

Alkaloids* / pharmacology
Antineoplastic Agents* / pharmacology
Cell Proliferation
Indole Alkaloids / pharmacology
Plant Extracts / pharmacology
Vinca*

Substances

Alkaloids
Indole Alkaloids
Antineoplastic Agents
Plant Extracts

Grants and funding

This research received no external funding.