The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence

F1000Res. 2023 Mar 29:12:348. doi: 10.12688/f1000research.132249.1. eCollection 2023.

Abstract

Profilin-1, a member of the Profilin family, is a ubiquitously expressed protein that controls actin polymerization in a concentration-dependent manner. As mutations in the Profilin-1 gene have potential implications in neurodegenerative disease progression, well-characterized anti-Profilin-1 antibodies would be beneficial to the scientific community. In this study, we characterized sixteen Profilin-1 commercial antibodies for Western blot, immunoprecipitation, and immunofluorescence applications, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

Keywords: PFN1; Profilin-1; Uniprot ID P07737; Western blot; antibody characterization; antibody validation; immunofluorescence; immunoprecipitation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / genetics
  • Blotting, Western
  • Fluorescent Antibody Technique
  • Humans
  • Immunoprecipitation
  • Mutation
  • Neurodegenerative Diseases*

Substances

  • Antibodies

Grants and funding